Expand Long Template Pcr System Roche

Not for use in diagnostic procedures.

Expand long template pcr system roche. Expand long template pcr system is a special enzyme mix that con tains thermostable taq dna polymerase and tgo dna polymerase 1 a thermostable dna polymerase with proofreading activity. This powerful polymerase mixture produces a high yield of pcr product from genomic dna. Expand long template pcr system is optimized to efficiently amplify. A hot start was then performed by maintaining the temperature in the heating block at 80 c until 1 75 u of the taq pwo enzyme mixture expand long template pcr system. 11 759 060 001 3 600 u 10 360 u version september 2005.

Roche applied science for general laboratory use. The pcr was carried out using expand long template pcr system kit roche diagnostics with 300 nmol l sense and anti sense primers and 50 ng cdna or genomic dna. For further processing on its own or in a mixture as part of an ivd method and under controlled conditions only acc. 11 681 834 001 150 u cat. Each sample was then overlaid with 2 drops of uv irradiated mineral oil sigma aldrich.

Roche applied science for general laboratory use. Expand long template pcr system deoxynucleoside triphosphate. Overview recombinant proteinase k in pcr grade quality is a universal tool for nucleic acid template preparation. Pcr amplification of long templates from nucleic acid samples obtained with the high pure pcr template preparation kit. Proteinase k originally isolated from the mold tritirachium album is a recombinant enzyme expressed in pichia pastoris it is a highly active subtilisin related serine endopeptidases that does not exhibit any pronounced cleavage specificity.

250 ng of each preparation was amplified using a tissue plasminogen activator tpa specific primer and either the expand long template pcr system. Expand long template pcr system. 11 681 842 001 720 u 2 360 u cat. 11 681 834 001 150 u cat. For in vitro use only.

11 759 060 001 3 600 u 10 360 u version september 2005. 11 681 842 001 720 u 2 360 u cat. Long range pcr to recover longer l1 insertions was performed with the expand long template pcr system roche according to the manufacturer s instructions in buffer 1 with 1 μl of 20 μm fs and es primers each and 25 ng of tumor dna. 5 junctions were pcr amplified using the same conditions as for the 3 junction except that a primer. Nucleic acids were prepared from blood or cultured human k562 cells.

For customers in the european economic area. Use this optimized enzyme blend and specially developed three buffer set to generate pcr products from 5 to 20kb in length. Not for use in diagnostic procedures. The amplified gfap utr amplicon was digested with ecori and xhoi before ligating the insert into pezx mt01 plasmid containing the luciferase reporter gene genecopoeia rockville md. For in vitro use only.

The main application of the expand long template pcr system is the accurate amplification of very long target sequences even from genomic dna.